Proteins are biologically active macromolecules with complex and sophisticated structures required to perform many essential cellular functions. The controlled and integrated actions of genes in living cells are used to convert the information encoded in DNA into a protein, which is then translated into a functional form using cellular machinery. The resulting protein is referred to as a recombinant protein.Read more

Recombinant proteins can be produced from DNA templates or from mammalian cells by a wide variety of expression systems. In order to ensure that the recombinant protein will function properly, it is necessary to consider factors such as molecular folding, post-translational modifications and proper attachment of a tag or other functional groups.

Streamlining Your Protein Production with Expert Expression Services

Bacterial protein expression systems are popular because they are easy to culture, grow fast and produce high yields of recombinant protein. However, quality concerns arise when expressing multi-domain or transmembrane proteins that require signal peptides and/or post-translational modifications like glycosylation. In addition, proteins expressed in bacteria often form insoluble aggregates called inclusion bodies that cannot be recovered without harsh denaturants and cumbersome protein-refolding procedures.

Mammalian cell-based systems offer a high level of protein production that is more functionally similar to native mammalian proteins. However, the availability of mammalian cell lines for large-scale characterization has been limited by cost and culture conditions. Recently, a series of Gateway-compatible baculovirus mediated insect cell expression systems have been developed that allow high-throughput construction of expression clones. With AMSBIO’s one-stop protein expression service, researchers can easily build and test multiple expression clones and select the best option to obtain high-quality recombinant proteins for use in their research.